Lipase Derived from Bacteria Isolated from The Seawater of Muara Badak, East Kalimantan
by Djihan Ryn Pratiwi, Safridah Hanum Nasution, Winni Astuti
Published: November 10, 2025 • DOI: 10.47772/IJRISS.2025.910000271
Abstract
Halophilic bacteria are extremophilic microorganisms that can optimally grow in environments with high salt concentrations. Sea water is one of the habitats of halobacteria. Isolation, characterization, and determination of the activity of crude extracts of lipase under various working conditions, the effect of metal ions, and variation of NaCl concentrations were performed. Lipase activity was determined using titrimetry. Four bacterial isolates were obtained from seawater Muara Badak, East Kalimantan, which showed fluorescent orange around the colony under UV light, with the highest activity in isolate 5 at 2.86 U/mL. The optimum working conditions of the crude extract of lipase were pH 6, temperature of 50°C, and substrate concentration of 2%.